18. Train (band)
17. Snowpiercer
16. Polar Express, movie version
15. Little plastic trains, Ticket to Ride
14. Darjeeling Limited
13. Shy Guy’s Perplex Express
12. Link’s trains, Spirit Tracks
11. Mrs. Cabobble’s Caboose
10. Orient Express
9. Thomas
8. Polar Express, book version
7. Demon Train, Spirit Tracks
6. Charge Man, Mega Man V
5. Quad City DJ’s C’Mon ‘N Ride It (The Train)
4. Hogwarts Express
3. Midnight Train to Georgia
2. Phantom Train, Final Fantasy VI
1. Soul Train
Chance Lee 
Big YES’s to 1 & 3, though I’m hoping that 18 just means that you’ve exhausted every other pop culture train in existence.
Yes! If the list is updated, I feel Train will always be at the bottom of the list.
What about the Marrakesh Express?